catalog number :
MBS913334
products type :
ELISA Kit
products full name :
Mouse Small proline-rich protein 2A, SPRR2A ELISA Kit
products short name :
small proline-rich protein 2A
products name syn :
Mouse Small proline-rich protein 2A (SPRR2A) ELISA kit; small proline-rich protein 2A
other names :
small proline-rich protein 2A2; Small proline-rich protein 2A; small proline-rich protein 2A2; small proline-rich protein 2A2
products gene name :
SPRR2A
other gene names :
Sprr2a2; Sprr2a
uniprot entry name :
SPR2A_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SPRR2A. No significant cross-reactivity or interference between mouse SPRR2A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: The minimum detectable dose of mouse SPRR2A is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPRR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001158259.1
ncbi gb acc num :
NM_001164787.1
ncbi mol weight :
9,408 Da
uniprot summary :
SPRR2A: Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Belongs to the cornifin (SPRR) family. During squamous differentiation of epidermal keratinocytes
size4 :
10x96-Strip-Wells