product summary
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company name :
MyBioSource
product type :
antibody
product name :
CHCHD4 Polyclonal Antibody
catalog :
MBS9133187
quantity :
0.05 mL
price :
200 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using CHCHD4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
product information
catalog number :
MBS9133187
products type :
Antibody
products full name :
CHCHD4 Polyclonal Antibody
products short name :
[CHCHD4]
products name syn :
[MIA40; TIMM40]
other names :
[mitochondrial intermembrane space import and assembly protein 40 isoform 1; Mitochondrial intermembrane space import and assembly protein 40; mitochondrial intermembrane space import and assembly protein 40; coiled-coil-helix-coiled-coil-helix domain containing 4; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4]
products gene name :
[CHCHD4]
other gene names :
[CHCHD4; CHCHD4; MIA40; TIMM40; MIA40]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse
sequence length :
142
sequence :
MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEE
HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYS
TEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEERE
KKPAEQAEETAPIEATATKEEEGSS
HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYS
TEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEERE
KKPAEQAEETAPIEATATKEEEGSS
purity :
Affinity Purification
storage stability :
Store at -20°C. Avoid freeze / thaw cycles.
tested application :
Western Blot (WB)
app notes :
WB: 1:500 - 1:2000
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_001091972.1)
other info2 :
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Calculated MW: 15kDa/17kDa. Observed MW: 20kDa
products categories :
Polyclonal Antibodies
ncbi gi num :
148612859
ncbi acc num :
NP_001091972.1
ncbi gb acc num :
NM_001098502.1
uniprot acc num :
Q8N4Q1
ncbi pathways :
Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261)
ncbi summary :
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008]
uniprot summary :
Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS) (PubMed:16185709, PubMed:19182799, PubMed:21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria (PubMed:16185709, PubMed:19182799, PubMed:21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
275
size3 :
0.2 mL
price3 :
430
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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