ELISA/assay

Human 26S proteasome non-ATPase regulatory subunit 4, PSMD4 ELISA Kit

MBS913293

96 Strip Wells

760 USD

ELISA Kit

Human 26S proteasome non-ATPase regulatory subunit 4, PSMD4 ELISA Kit

proteasome (prosome, macropain) 26S subunit, non-ATPase, 4

Human 26S proteasome non-ATPase regulatory subunit 4 (PSMD4) ELISA kit; RP11-126K1.1; AF; AF-1; ASF; MCB1; Rpn10; S5A; pUB-R5; 26S protease subunit S5a; 26S proteasome non-ATPase regulatory subunit 4; S5a/antisecretory factor protein; angiocidin; antisecretory factor 1; mult; proteasome (prosome; macropain) 26S subunit; non-ATPase; 4

26S proteasome non-ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; angiocidin; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; Antisecretory factor 1; AF; ASF; Multiubiquitin chain-binding protein

PSMD4

PSMD4; PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5; MCB1; AF; ASF

PSMD4_HUMAN

Human

377

This assay has high sensitivity and excellent specificity for detection of Human PSMD4. No significant cross-reactivity or interference between Human PSMD4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 34.4 pg/ml-2200 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 8.6 pg/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSMD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSMD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD4 bound in the initial step. The color development is stopped and the intensity of the color is measured

5292161

NP_002801.1

NM_002810.2

P55036

40,737 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Securin Pathway 105833!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105829!!Activation Of NF-kappaB In B Cells Pathway 576251!!Adaptive Immune System Pathway 366160!!Antigen Processing-Cross Presentation Pathway 477122!!Antigen Processing: Ubiquitination Proteasome Degradation Pathway 366162!!Apoptosis Pathway 105648

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

Function: Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.

96 Strip Wells

760 USD