catalog number :
MBS913274
products type :
ELISA Kit
products full name :
Bovine C-C motif chemokine 8, CCL8 ELISA Kit
products short name :
chemokine (C-C motif) ligand 8
products name syn :
Bovine C-C motif chemokine 8 (CCL8) ELISA kit; HC14; MCP-2; MCP2; SCYA10; SCYA8; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys); member 8 (monocyte che; chemokine (C-C motif) ligand 8
other names :
C-C motif chemokine 8; C-C motif chemokine 8; C-C motif chemokine 8; MCP-2; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); chemokine (C-C motif) ligand 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8
products gene name :
CCL8
other gene names :
CCL8; CCL8; SCYA8; MCP2; SCYA8; MCP-2
uniprot entry name :
CCL8_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine CCL8. No significant cross-reactivity or interference between Bovine CCL8 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776432.1
ncbi gb acc num :
NM_174007.1
ncbi mol weight :
10,900 Da
ncbi pathways :
Chemokine Signaling Pathway (98761); Chemokine Signaling Pathway (96864)
uniprot summary :
Function: Chemotactic factor that attracts monocytes. This protein can bind heparin.
size4 :
10x96-Strip-Wells