Human lactate dehydrogenase A, LDHA ELISA Kit | MyBioSource MBS913194 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human lactate dehydrogenase A, LDHA ELISA Kit

catalog :

MBS913194

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS913194

products type :

ELISA Kit

products full name :

Human lactate dehydrogenase A, LDHA ELISA Kit

products short name :

lactate dehydrogenase A

products name syn :

Human lactate dehydrogenase A (LDHA) ELISA kit; GSD11; LDH1; LDHM; PIG19; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; lactate dehydrogenase A

other names :

L-lactate dehydrogenase A chain isoform 2; L-lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; LDH-A; LDH-M; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; lactate dehydrogenase A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59

products gene name :

LDHA

other gene names :

LDHA; LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P; PIG19; LDH-A; LDH-M

uniprot entry name :

LDHA_HUMAN

reactivity :

Human

sequence length :

274

specificity :

This assay has high sensitivity and excellent specificity for detection of human LDHA. No significant cross-reactivity or interference between human LDHA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 7.81 mU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

207028494

ncbi acc num :

NP_001128711.1

ncbi gb acc num :

NM_001135239.1

uniprot acc num :

P00338

ncbi mol weight :

36,689 Da

ncbi pathways :

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198814); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Pathway (477135); Propanoate Metabolism Pathway (83004); Propanoate Metabolism Pathway (387); Pyruvate Metabolism Pathway (106205)

ncbi summary :

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

uniprot summary :

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Carbohydrate Metabolism - propanoate; EC 1.1.1.27; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - cysteine and methionine. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: membrane; mitochondrion; cytosol; nucleus. Molecular Function: protein binding; L-lactate dehydrogenase activity. Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process. Disease: Glycogen Storage Disease Xi

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage