HTRA1 Polyclonal Antibody | MyBioSource MBS9131684 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HTRA1 Polyclonal Antibody

catalog :

MBS9131684

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using HTRA1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

product information

catalog number :

MBS9131684

products type :

Antibody

products full name :

HTRA1 Polyclonal Antibody

products short name :

[HTRA1]

products name syn :

[ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11]

other names :

[Serine protease HTRA1; Serine protease HTRA1; serine protease HTRA1; HtrA serine peptidase 1; High-temperature requirement A serine peptidase 1; L56; Serine protease 11]

products gene name :

[HTRA1]

other gene names :

[HTRA1; HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2; HTRA; PRSS11]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat

sequence length :

480

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB: 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acid 22-160 of human HTRA1 (NP_002766.1). Calculated MW: 51 kDa. Observed MW: 55 kDa

other info2 :

Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Primary antibody

products description :

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7.

ncbi gi num :

18202620

ncbi acc num :

Q92743.1

uniprot acc num :

Q92743

ncbi summary :

uniprot summary :

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family. Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q26.13. Cellular Component: extracellular matrix; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: extracellular matrix disassembly; proteolysis. Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy, Type 2; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy; Macular Degeneration, Age-related, 7

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage