PRKCA Monoclonal Antibody | MyBioSource MBS9131320 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PRKCA Monoclonal Antibody

catalog :

MBS9131320

quantity :

0.05 mL

price :

275 USD

clonality :

monoclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using PRKCA antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunohistochemistry of paraffin-embedded mouse brain using PRKCA antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded human lung using PRKCA antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9131320

products type :

Antibody

products full name :

PRKCA Monoclonal Antibody

products short name :

[PRKCA]

products name syn :

[AAG6; PKC-alpha; PKCA; PRKACA]

other names :

[Protein kinase C alpha type; Protein kinase C alpha type; protein kinase C alpha type; protein kinase C alpha]

products gene name :

[PRKCA]

other gene names :

[PRKCA; PRKCA; AAG6; PKCA; PRKACA; PKC-alpha; PKCA; PRKACA; PKC-A; PKC-alpha]

clonality :

Monoclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

672

purity :

Affinity Purification

storage stability :

Store at -20 degree C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded mouse intestin using PRKCA antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded mouse heart using PRKCA antibody at dilution of 1:100 (40x lens).

other info1 :

Source: Rabbit. Immunogen: Recombinant protein of human PRKCA

other info2 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Primary antibody

products description :

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

ncbi gi num :

317373571

ncbi acc num :

P17252.4

uniprot acc num :

P17252

ncbi mol weight :

77kDa

ncbi pathways :

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Regulates Insulin Secretion Pathway (105924); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807)

ncbi summary :

uniprot summary :

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators. Protein type: AGC group; Alpha subfamily; EC 2.7.11.13; Kinase, protein; Oncoprotein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor). Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; plasma membrane. Molecular Function: calcium-dependent protein kinase C activity; enzyme binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity. Biological Process: adenylate cyclase activation; axon guidance; mitotic nuclear envelope disassembly; negative regulation of adenylate cyclase activity; peptidyl-serine phosphorylation; platelet activation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of insulin secretion; regulation of mRNA stability

size1 :

0.05 mL

price1 :

275 USD

size2 :

0.1 mL

price2 :

430

size3 :

0.2 mL

price3 :

655

more info or order :

MyBioSource product webpage