catalog number :
MBS913118
products type :
ELISA Kit
products full name :
Rat Protein S100-A10, S100A10 ELISA Kit
products short name :
S100 calcium binding protein A10
products name syn :
Rat Protein S100-A10 (S100A10) ELISA kit; 42C; ANX2L; ANX2LG; CAL1L; CLP11; Ca[1]; GP11; MGC111133; P11; p10; OTTHUMP00000015270; S100 calcium binding protein A10 (annexin II ligand; calpactin I; light polypeptide (p11) ) ; S100 calcium-binding; S100 calcium binding protein A10
other names :
protein S100-A10; Protein S100-A10; protein S100-A10; p10 protein; calpactin I light chain; calpactin-1 light chain; S100 calcium-binding protein A10; Calpactin I light chain; Calpactin-1 light chain; S100 calcium-binding protein A10; p10 protein
products gene name :
S100A10
other gene names :
S100A10; S100a10
uniprot entry name :
S10AA_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat S100A10. No significant cross-reactivity or interference between rat S100A10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 40 ng/ml. Sensitivity: The minimum detectable dose of rat S100A10 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075632.1
ncbi gb acc num :
NM_001082163.1
ncbi mol weight :
11,203 Da
uniprot summary :
Function: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase
size4 :
10x96-Strip-Wells