ELISA/assay

Human Transient receptor potential Cation channel subfamily V member 6, TRPV6 ELISA Kit

MBS913109

48-Strip-Wells

510 USD

ELISA Kit

Human Transient receptor potential Cation channel subfamily V member 6, TRPV6 ELISA Kit

transient receptor potential cation channel, subfamily V, member 6

Human Transient receptor potential cation channel subfamily V member 6 (TRPV6) ELISA kit; ABP/ZF; CAT1; CATL; ECAC2; HSA277909; LP6728; ZFAB; Alu-binding protein with zinc finger domain; calcium channel CaT1; epithelial apical membrane calcium transporter/channel CaT1; epithelial calcium ch; transient receptor potential cation channel; subfamily V; member 6

transient receptor potential cation channel subfamily V member 6; Transient receptor potential cation channel subfamily V member 6; transient receptor potential cation channel subfamily V member 6; calcium transport protein 1; epithelial calcium channel 2; calcium transporter-like protein; Alu-binding protein with zinc finger domain; epithelial apical membrane calcium transporter/channel CaT1; transient receptor potential cation channel, subfamily V, member 6; CaT-like; CaT-L; Calcium transport protein 1; CaT1; Epithelial calcium channel 2; ECaC2

TRPV6

TRPV6; TRPV6; CAT1; CATL; ZFAB; ECAC2; ABP/ZF; LP6728; HSA277909; ECAC2; TrpV6; CaT-L; CaT1; ECaC2

TRPV6_HUMAN

Human

725

This assay has high sensitivity and excellent specificity for detection of human TRPV6. No significant cross-reactivity or interference between human TRPV6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human TRPV6 is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRPV6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRPV6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPV6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

526479841

NP_061116.3

NM_018646.4

Q9H1D0

83,210 Da

Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Signaling Events Mediated By PTP1B Pathway (138017); TCR Signaling In Naive CD4+ T Cells Pathway (137998); TCR Signaling In Naive CD8+ T Cells Pathway (138055)

This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds. [provided by RefSeq, Jul 2013]

TRPV6: Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin- dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7q34. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: calmodulin binding; protein binding; calcium channel activity. Biological Process: calcium ion transport; transmembrane transport; regulation of calcium ion-dependent exocytosis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800