product summary
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company name :
MyBioSource
product type :
antibody
product name :
LGMN Polyclonal Antibody
catalog :
MBS9131059
quantity :
0.05 mL
price :
200 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using LGMN antibody (MBS9131059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
image 2 :
Immunofluorescence analysis of C6 cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
image 3 :
Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
product information
catalog number :
MBS9131059
products type :
Antibody
products full name :
LGMN Polyclonal Antibody
products short name :
[LGMN]
products name syn :
[LGMN; AEP; LGMN1; PRSC1; legumain]
other names :
[Legumain; Legumain; legumain; legumain; Asparaginyl endopeptidase; Protease, cysteine 1]
products gene name :
[LGMN]
other gene names :
[LGMN; LGMN; AEP; LGMN1; PRSC1; PRSC1]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse, Rat
purity :
Affinity Purification
form :
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
storage stability :
Store at -20 degree C. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB), Immunofluorescence (IF)
app notes :
Recommended Dilutions: WB: 1:1000 - 1:2000, IF: 1:50 - 1:200
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence (IF)
image3 heading :
Immunofluorescence (IF)
image4 heading :
Immunofluorescence (IF)
image4 description :
Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody (MBS9131059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
other info1 :
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1).
products categories :
Primary antibody
products description :
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
ncbi gi num :
2842759
ncbi acc num :
Q99538.1
uniprot acc num :
Q99538
ncbi mol weight :
Observed MW: 54kDa. Calculated MW: 42kDa/49kDa
ncbi pathways :
Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Lysosome Pathway (99052); Lysosome Pathway (96865); MHC Class II Antigen Presentation Pathway (1269200); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147)
ncbi summary :
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]
uniprot summary :
LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family. Protein type: EC 3.4.22.34; Protease. Chromosomal Location of Human Ortholog: 14q32.12. Cellular Component: lysosomal lumen; lysosome. Molecular Function: cysteine-type endopeptidase activity; peptidase activity. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
275
size3 :
0.2 mL
price3 :
430
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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