CA9 Polyclonal Antibody | MyBioSource MBS9129810 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CA9 Polyclonal Antibody

catalog :

MBS9129810

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using CA9 antibody at 1:100 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

product information

catalog number :

MBS9129810

products type :

Antibody

products full name :

CA9 Polyclonal Antibody

products short name :

[CA9]

products name syn :

[MN; CAIX]

other names :

[carbonic anhydrase 9; Carbonic anhydrase 9; carbonic anhydrase 9; carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1]

products gene name :

[CA9]

other gene names :

[CA9; CA9; MN; CAIX; G250; MN; CA-IX; CAIX; RCC-associated antigen G250]

uniprot entry name :

CAH9_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Mouse, Rat

sequence length :

459

purity :

Affinity purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50-1:200

image1 heading :

Western Blot (WB)

other info1 :

Source: Rabbit. Immunogen: Recombinant protein of human CA9

other info2 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

ncbi gi num :

169636420

ncbi acc num :

NP_001207.2

ncbi gb acc num :

NM_001216.2

uniprot acc num :

Q16790

ncbi mol weight :

50kDa

ncbi pathways :

Cellular Response To Hypoxia Pathway (1270415); Cellular Responses To Stress Pathway (1270414); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Pathway (1269956); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (1270416); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (1270419); Reversible Hydration Of Carbon Dioxide Pathway (1270229)

ncbi summary :

uniprot summary :

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: Nucleolus; Membrane protein, integral; EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: basolateral plasma membrane; integral to membrane; microvillus membrane; nucleolus; plasma membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to drug; response to testosterone stimulus; secretion

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage