NACA Polyclonal Antibody | MyBioSource MBS9129510 product information

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company name :

MyBioSource

product type :

antibody

product name :

NACA Polyclonal Antibody

catalog :

MBS9129510

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NACA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

product information

catalog number :

MBS9129510

products type :

Antibody

products full name :

NACA Polyclonal Antibody

products short name :

[NACA]

products name syn :

[NACA; HSD48; NAC-alpha; NACA1; skNAC; nascent polypeptide-associated complex subunit alpha]

other names :

[nascent polypeptide-associated complex subunit alpha isoform X3; Nascent polypeptide-associated complex subunit alpha, muscle-specific form; nascent polypeptide-associated complex subunit alpha; nascent polypeptide-associated complex alpha subunit; Alpha-NAC, muscle-specific form; skNAC]

products gene name :

[NACA]

other gene names :

[NACA; NACA; HSD48; NACA1; skNAC; NAC-alpha; skNAC]

uniprot entry name :

NACAM_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

2078

purity :

Affinity purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20 degree C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:100. IP: 1:50-1:100

image1 heading :

Western Blot (WB)

other info1 :

Source: Rabbit. Immunogen: Recombinant protein of human NACA. Category: Primary Antibody

products categories :

Polyclonal

products description :

This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.

ncbi gi num :

767974358

ncbi acc num :

XP_011536691.1

ncbi gb acc num :

XM_011538389.1

uniprot acc num :

E9PAV3

ncbi mol weight :

Calculated MW: 23kDa/94kDa/205kDa. Observed MW: 37kDa

ncbi summary :

This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]

uniprot summary :

NACA: Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. Belongs to the NAC-alpha family. Interacts with TBP and JUN. Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains. Interacts with ASFV protein H339R. Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q23-q24.1. Cellular Component: cytoplasm; nascent polypeptide-associated complex; nucleus. Molecular Function: DNA binding; protein binding; transcription coactivator activity. Biological Process: positive regulation of skeletal muscle growth; protein transport; regulation of skeletal muscle fiber development; regulation of transcription, DNA-dependent; skeletal muscle regeneration; transcription, DNA-dependent; translation; viral reproduction

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage