product summary
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company name :
MyBioSource
product type :
antibody
product name :
NPL Polyclonal Antibody
catalog :
MBS9129404
quantity :
0.05 mL
price :
200 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using NPL antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
product information
catalog number :
MBS9129404
products type :
Antibody
products full name :
NPL Polyclonal Antibody
products short name :
[NPL]
products name syn :
[C112; C1orf13; NAL; NPL1]
other names :
[N-acetylneuraminate lyase isoform 2; N-acetylneuraminate lyase; N-acetylneuraminate lyase; N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase); N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialate-pyruvate lyase; Sialic acid aldolase; Sialic acid lyase]
products gene name :
[NPL]
other gene names :
[NPL; NPL; NAL; C112; NPL1; C1orf13; C1orf13; NALase]
uniprot entry name :
NPL_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Mouse, Rat
sequence length :
301
purity :
Affinity purification
form :
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
storage stability :
Store at -20°C. Avoid freeze / thaw cycles.
tested application :
Western Blot (WB)
app notes :
WB: 1:200 - 1:2000
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NPL (NP_110396.1)
other info2 :
Calculated MW: 12 kDa/26 kDa/31 kDa/33 kDa/35 kDa. Observed MW: 37 kDa
products categories :
Polyclonal
ncbi gi num :
318037618
ncbi acc num :
NP_001186979.1
ncbi gb acc num :
NM_001200050.1
uniprot acc num :
Q9BXD5
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Asparagine N-linked Glycosylation Pathway (1268714); Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway (1268715); Metabolism Of Proteins Pathway (1268677); Post-translational Protein Modification Pathway (1268701); Sialic Acid Metabolism Pathway (1268721); Synthesis Of Substrates In N-glycan Biosythesis Pathway (1268716)
ncbi summary :
This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
uniprot summary :
NPL: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Belongs to the DHDPS family. NanA subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Lyase; EC 4.1.3.3. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: cytosol. Molecular Function: N-acetylneuraminate lyase activity; protein binding. Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; N-acetylneuraminate catabolic process; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
275
size3 :
0.2 mL
price3 :
430
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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