PATZ1 Polyclonal Antibody | MyBioSource MBS9129054 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PATZ1 Polyclonal Antibody

catalog :

MBS9129054

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using PATZ1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

product information

catalog number :

MBS9129054

products type :

Antibody

products full name :

PATZ1 Polyclonal Antibody

products short name :

[PATZ1]

products name syn :

[PATZ1; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; ZSG;dJ400N23; POZ/BTB and AT hook containing zincfinger 1]

other names :

[POZ-, AT hook-, and zinc finger-containing protein 1 long C isoform; POZ-, AT hook-, and zinc finger-containing protein 1; POZ-, AT hook-, and zinc finger-containing protein 1; POZ/BTB and AT hook containing zinc finger 1; BTB/POZ domain zinc finger transcription factor; Protein kinase A RI subunit alpha-associated protein; Zinc finger and BTB domain-containing protein 19; Zinc finger protein 278; Zinc finger sarcoma gene protein]

products gene name :

[PATZ1]

other gene names :

[PATZ1; PATZ1; ZSG; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; dJ400N23; PATZ; RIAZ; ZBTB19; ZNF278; ZSG]

uniprot entry name :

PATZ1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

687

purity :

Affinity purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB: 1:1000 - 1:4000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1(NP_114440.1). Calculated MW: 57kDa/69kDa/74kDa. Observed MW: 74kDa

other info2 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q then the hybrid thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein . This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.

ncbi gi num :

14670360

ncbi acc num :

NP_055138.2

ncbi gb acc num :

NM_014323.2

uniprot acc num :

Q9HBE1

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085)

ncbi summary :

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PATZ1: Transcriptional repressor. A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 22q12.2. Cellular Component: nucleoplasm; nucleus. Molecular Function: chromatin binding; DNA binding; metal ion binding. Biological Process: male gonad development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatogenesis; T cell differentiation; transcription from RNA polymerase II promoter

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage