Phospho-RAF1-S259 Polyclonal Antibody | MyBioSource MBS9128987 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-RAF1-S259 Polyclonal Antibody

catalog :

MBS9128987

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of HeLa cells, using Phospho-RAF1-S259 antibody (MBS9128987) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

image 2 :

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (MBS9128987). Western blot was performed from the immunoprecipitate using Phospho- RAF1-S259 pAb (MBS9128987) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.

product information

catalog number :

MBS9128987

products type :

Antibody

products full name :

Phospho-RAF1-S259 Polyclonal Antibody

products short name :

[Phospho-RAF1-S259]

products name syn :

[RAF1; CMD1NN; CRAF; NS5; Raf-1; c-Raf; Raf-1 p roto-oncogene, serine/threonine kinase]

other names :

[RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1 proto-oncogene, serine/threonine kinase; Proto-oncogene c-RAF; cRaf; Raf-1]

products gene name :

[RAF1-S259]

other gene names :

[RAF1; RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF; cRaf]

uniprot entry name :

RAF1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse

sequence length :

648

purity :

Affinity purification

form :

PBS with 50% glycerol, pH7.3.

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunoprecipitation (IP)

app notes :

WB 1:500 - 1:2000. IP 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunoprecipitation (IP0

other info1 :

Immunogen: A synthetic phosphorylated peptide around S259 of human RAF1 (NP_002871.1).

products categories :

Phosphorylated

products description :

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

ncbi gi num :

4506401

ncbi acc num :

NP_002871.1

ncbi gb acc num :

NM_002880.3

uniprot acc num :

P04049

ncbi mol weight :

Observed MW: 73kDa. Calculated MW: 73kDa/75kDa

ncbi pathways :

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (1269471); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1268798); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Apoptosis Pathway (83060); Apoptosis Pathway (470)

ncbi summary :

uniprot summary :

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage