Phospho-FYN-Y416/Lck-Y416 pAb | MyBioSource MBS9128730 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-FYN-Y416/Lck-Y416 pAb

catalog :

MBS9128730

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of NIH3T3 cells, using Phospho-FYN-Y416/Lck-Y416 antibody (MBS9128730). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

product information

catalog number :

MBS9128730

products type :

Antibody

products full name :

Phospho-FYN-Y416/Lck-Y416 pAb

products short name :

[FYN-Y420]

other names :

[tyrosine-protein kinase Fyn isoform a; Tyrosine-protein kinase Fyn; tyrosine-protein kinase Fyn; FYN proto-oncogene, Src family tyrosine kinase; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn]

products gene name :

[FYN]

other gene names :

[FYN; FYN; SLK; SYN; p59-FYN; SLK]

uniprot entry name :

FYN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

537

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Category: Phosphorylated Antibodies

other info2 :

Immunogen: A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family

ncbi gi num :

4503823

ncbi acc num :

NP_002028.1

ncbi gb acc num :

NM_002037.5

uniprot acc num :

P06241

ncbi mol weight :

Calculated MW: 54kDa/60kDa/40kDa/58kDa/61kDa. Observed MW: 61kDa

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Adaptive Immune System Pathway (1269171); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha-synuclein Signaling Pathway (137913); Alpha6-Beta4 Integrin Signaling Pathway (198807); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway (1269184); Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

ncbi summary :

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

uniprot summary :

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy. Protein type: Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Src family. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; cytosol; nucleus; endosome; lipid raft. Molecular Function: tubulin binding; CD8 receptor binding; ephrin receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; G-protein-coupled receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; T cell receptor binding; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding. Biological Process: axon guidance; central nervous system development; nerve growth factor receptor signaling pathway; viral reproduction; T cell activation; neuron migration; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; blood coagulation; negative regulation of protein catabolic process; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage