ABCE1 Polyclonal Antibody | MyBioSource MBS9128630 product information

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company name :

MyBioSource

product type :

antibody

product name :

ABCE1 Polyclonal Antibody

catalog :

MBS9128630

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using ABCE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

image 2 :

Immunofluorescence analysis uf U2OS cells using ABCE1 Antibody MBS9128630 at dilution of 1:100 . Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9128630

products type :

Antibody

products full name :

ABCE1 Polyclonal Antibody

products short name :

[ABCE1]

products name syn :

[ABC38,OABP,RLI,RLI1,RNASEL1,RNASELI,RNS4I]

other names :

[ATP-binding cassette sub-family E member 1; ATP-binding cassette sub-family E member 1; ATP-binding cassette sub-family E member 1; ATP-binding cassette, sub-family E (OABP), member 1; 2'-5'-oligoadenylate-binding protein; HuHP68; RNase L inhibitor; Ribonuclease 4 inhibitor; RNS4I]

products gene name :

[ABCE1]

other gene names :

[ABCE1; ABCE1; RLI; OABP; ABC38; RNS4I; RNASEL1; RNASELI; RLI; RNASEL1; RNASELI; RNS4I; RNS4I]

uniprot entry name :

ABCE1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

599

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

WB, IF

app notes :

WB 1:500 - 1:2000. IF 1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence IF

other info1 :

Calculated MW: 67 kDa. Observed MW: 67 Kda . Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 320-599 of human ABCE1 (NP_002931.2)

other info2 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products description :

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.

ncbi gi num :

108773784

ncbi acc num :

NP_001035809.1

ncbi gb acc num :

NM_001040876.1

uniprot acc num :

P61221

ncbi pathways :

ABC-family Proteins Mediated Transport Pathway (1269904); Transmembrane Transport Of Small Molecules Pathway (1269903)

ncbi summary :

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ABCE1: Antagonizes the binding of 2-5A (5 -phosphorylated 2 ,5 -linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. Belongs to the ABC transporter superfamily. ABCE family. Chromosomal Location of Human Ortholog: 4q31. Cellular Component: mitochondrion; membrane; cytoplasm. Molecular Function: ATPase activity; iron-sulfur cluster binding; ribonuclease inhibitor activity; ATP binding. Biological Process: viral reproduction; response to virus; RNA catabolic process; negative regulation of catalytic activity

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage