EXO1 Polyclonal Antibody | MyBioSource MBS9128501 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EXO1 Polyclonal Antibody

catalog :

MBS9128501

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using EXO1 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

image 2 :

Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded human lung cancer using EXO1 antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9128501

products type :

Antibody

products full name :

EXO1 Polyclonal Antibody

products short name :

[EXO1]

products name syn :

[EXO1; HEX1; hExoI; exonuclease 1]

other names :

[exonuclease 1 isoform a; Exonuclease 1; exonuclease 1; exonuclease 1; Exonuclease I; hExoI]

products gene name :

[EXO1]

other gene names :

[EXO1; EXO1; HEX1; hExoI; EXOI; HEX1; hExo1; hExoI]

uniprot entry name :

EXO1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

803

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:100. IF: 1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded human gastric cancer using EXO1 antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody at dilution of 1:100 (40x lens).

other info1 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).

products description :

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

ncbi gi num :

284055276

ncbi acc num :

NP_003677.4

ncbi gb acc num :

NM_003686.4

uniprot acc num :

Q9UQ84

ncbi mol weight :

Observed MW: 115kDa. Calculated MW: 89kDa/94kDa

ncbi pathways :

DNA Repair Pathway (1270350); Mismatch Repair Pathway (1270411); Mismatch Repair Pathway (198875); Mismatch Repair Pathway (83045); Mismatch Repair Pathway (453); Mismatch Repair (MMR) Directed By MSH2:MSH3 (MutSbeta) Pathway (1270413); Mismatch Repair (MMR) Directed By MSH2:MSH6 (MutSalpha) Pathway (1270412)

ncbi summary :

uniprot summary :

EXO1: 5 3 double-stranded DNA exonuclease which may also possess a cryptic 3 5 double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5 or 3 to the mismatch. Also exhibits endonuclease activity against 5 -overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5 -end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.1.-.-. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: nucleoplasm; nucleus. Molecular Function: 5 -3 exonuclease activity; protein binding; double-stranded DNA specific 5 -3 exodeoxyribonuclease activity; structure-specific DNA binding; DNA binding; 5 -3 exodeoxyribonuclease activity; single-stranded DNA specific 5 -3 exodeoxyribonuclease activity; metal ion binding; exonuclease activity; ribonuclease H activity; flap endonuclease activity. Biological Process: humoral immune response mediated by circulating immunoglobulin; mismatch repair; meiotic cell cycle; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage