IL5 Polyclonal Antibody | MyBioSource MBS9128390 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IL5 Polyclonal Antibody

catalog :

MBS9128390

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using IL5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

image 2 :

Immunohistochemistry of paraffin-embedded human liver using IL5 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded human stomach using IL5 antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9128390

products type :

Antibody

products full name :

IL5 Polyclonal Antibody

products short name :

[IL5]

other names :

[interleukin-5; Interleukin-5; interleukin-5; interleukin 5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF]

products gene name :

[IL5]

other gene names :

[IL5; IL5; EDF; TRF; IL-5; IL-5; TRF]

uniprot entry name :

IL5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

134

purity :

Affinity Purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB 1:500 - 1:2000. IHC 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Recombinant funsion protein containing a sequence Corresponsding to amino acids 20-134 of human IL5.

products description :

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2. This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

ncbi gi num :

4504671

ncbi acc num :

NP_000870.1

ncbi gb acc num :

NM_000879.2

uniprot acc num :

P05113

ncbi mol weight :

15kDa

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Axon Guidance Pathway (1270303); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

ncbi summary :

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

uniprot summary :

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family. Protein type: Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity. Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage