EHMT1 Polyclonal Antibody | MyBioSource MBS9128353 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EHMT1 Polyclonal Antibody

catalog :

MBS9128353

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using EHMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

product information

catalog number :

MBS9128353

products type :

Antibody

products full name :

EHMT1 Polyclonal Antibody

products short name :

[EHMT1]

products name syn :

[bA188C12.1; Eu-HmTase1; EUHMTASE1; FP13812; GLP; GLP1; KMT1D]

other names :

[histone-lysine N-methyltransferase EHMT1 isoform 2; Histone-lysine N-methyltransferase EHMT1; histone-lysine N-methyltransferase EHMT1; euchromatic histone-lysine N-methyltransferase 1; Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP; GLP1; Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Lysine N-methyltransferase 1D]

products gene name :

[EHMT1]

other gene names :

[EHMT1; EHMT1; GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1; EUHMTASE1; GLP; KIAA1876; KMT1D; Eu-HMTase1; GLP; GLP1; H3-K9-HMTase 5]

uniprot entry name :

EHMT1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

808

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Source: Rabbit. Calculated MW: 7 kDa/86 kDa/128 kDa/ 141 kDa. Observed MW: 141 kDa

other info2 :

Immunogen: Recombinant fusion protein containing a sequene corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4). Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products description :

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants.

ncbi gi num :

224465235

ncbi acc num :

NP_001138999.1

ncbi gb acc num :

NM_001145527.1

uniprot acc num :

Q9H9B1

ncbi pathways :

Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320); PKMTs Methylate Histone Lysines Pathway (1270438); Senescence-Associated Secretory Phenotype (SASP) Pathway (1270431)

ncbi summary :

uniprot summary :

EHMT1: Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; chromosome; nucleus. Molecular Function: methyltransferase activity; protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific). Biological Process: histone methylation; embryonic development; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; histone H3-K9 methylation. Disease: Kleefstra Syndrome

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage