MT-ND5 Polyclonal Antibody | MyBioSource MBS9128307 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MT-ND5 Polyclonal Antibody

catalog :

MBS9128307

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of mouse brain, using ND5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

image 2 :

Immunohistochemistry of paraffin-embedded rat brain using ND5 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded rat kidney using ND5 antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9128307

products type :

Antibody

products full name :

MT-ND5 Polyclonal Antibody

products short name :

[MT-ND5]

other names :

[NADH dehydrogenase subunit 5 (mitochondrion); NADH-ubiquinone oxidoreductase chain 5; NADH dehydrogenase, subunit 5 (complex I); mitochondrially encoded NADH dehydrogenase 5; NADH dehydrogenase subunit 5]

products gene name :

[ND5]

other gene names :

[MT-ND5; MT-ND5; MTND5; ND5; MTND5; NADH5; ND5]

uniprot entry name :

NU5M_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

603

purity :

Affinity Purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB)

app notes :

WB 1:500 - 1:2000

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded human colon carcinoma using ND5 antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded human prostate cancer using ND5 antibody at dilution of 1:100 (40x lens).

other info1 :

Route: Synthetic Peptide. Category: Primary antibody

other info2 :

Immunogen: A synthetic peptide of human MT-ND5

ncbi gi num :

251831117

ncbi acc num :

YP_003024036.1

ncbi gb acc num :

NC_012920.1

uniprot acc num :

P03915

ncbi mol weight :

67kDa

ncbi pathways :

Electron Transport Chain Pathway (198860); Metabolic Pathways (132956); Metabolism Pathway (1269956); NADH:ubiquinone Oxidoreductase, Mitochondria Pathway (413406); NADH:ubiquinone Oxidoreductase, Mitochondria Pathway (890440); Oxidative Phosphorylation Pathway (198870); Oxidative Phosphorylation Pathway (82942); Oxidative Phosphorylation Pathway (303); Parkinson's Disease Pathway (83098); Respiratory Electron Transport Pathway (1270128)

uniprot summary :

MT-ND5: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Defects in MT-ND5 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS). MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness. Belongs to the complex I subunit 5 family. Protein type: Mitochondrial; Membrane protein, multi-pass; Oxidoreductase; Membrane protein, integral; EC 1.6.5.3. Chromosomal Location of Human Ortholog: -. Disease: Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, And Stroke-like Episodes; Leber Optic Atrophy

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage