XRCC4 Polyclonal Antibody | MyBioSource MBS9128276 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

XRCC4 Polyclonal Antibody

catalog :

MBS9128276

quantity :

0.1 mL

price :

275 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of K-562 cells, using XRCC4 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

product information

catalog number :

MBS9128276

products type :

Antibody

products full name :

XRCC4 Polyclonal Antibody

products short name :

[XRCC4]

products name syn :

[SSMED]

other names :

[DNA repair protein XRCC4 isoform 1; DNA repair protein XRCC4; DNA repair protein XRCC4; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross-complementing protein 4]

products gene name :

[XRCC4]

other gene names :

[XRCC4; XRCC4]

uniprot entry name :

XRCC4_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human

sequence length :

334

purity :

Affinity Purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles

tested application :

Western Blot (WB)

app notes :

WB: 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Source: Rabbit. Category: Primary Antibody

other info2 :

Immunogen: A synthetic peptide of human XRCC4. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products description :

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants

ncbi gi num :

4507945

ncbi acc num :

NP_003392.1

ncbi gb acc num :

NM_003401.3

uniprot acc num :

Q13426

ncbi mol weight :

38kDa

ncbi pathways :

2-LTR Circle Formation Pathway (1269067); DNA Repair Pathway (1270350); Disease Pathway (1268854); Double-Strand Break Repair Pathway (1270380); Early Phase Of HIV Life Cycle Pathway (1269059); HIV Infection Pathway (1269057); HIV Life Cycle Pathway (1269058); Infectious Disease Pathway (1269056); Integration Of Provirus Pathway (1269065); Metabolism Of Proteins Pathway (1268677)

ncbi summary :

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008]

uniprot summary :

XRCC4: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. Widely expressed. Belongs to the XRCC4 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA repair, damage. Chromosomal Location of Human Ortholog: 5q14.2. Cellular Component: nucleoplasm; DNA ligase IV complex; centrosome; condensed chromosome; DNA-dependent protein kinase complex; cell junction; cytosol; nucleus. Molecular Function: protein C-terminus binding; protein binding; DNA binding; ligase activity. Biological Process: pro-B cell differentiation; positive regulation of neurogenesis; central nervous system development; immunoglobulin V(D)J recombination; viral reproduction; in utero embryonic development; T cell differentiation in the thymus; isotype switching; DNA repair; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; double-strand break repair; response to gamma radiation; DNA ligation during DNA repair; negative regulation of neuron apoptosis; positive regulation of ligase activity; response to X-ray. Disease: Short Stature, Microcephaly, And Endocrine Dysfunction

size1 :

0.1 mL

price1 :

275 USD

size2 :

0.2 mL

price2 :

430

more info or order :

MyBioSource product webpage