RAC1 Polyclonal Antibody | MyBioSource MBS9127955 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAC1 Polyclonal Antibody

catalog :

MBS9127955

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using RAC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

image 2 :

Immunohistochemistry of paraffin-embedded human gastric using RAC1 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunofluorescence analysis of A549 cells using RAC1 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9127955

products type :

Antibody

products full name :

RAC1 Polyclonal Antibody

products short name :

[RAC1]

products name syn :

[RAC1; MIG5; Rac-1; TC-25; p21-Rac1; ras-related C3 botulinum toxin substrate 1]

other names :

[ras-related C3 botulinum toxin substrate 1 isoform Rac1; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1]

products gene name :

[RAC1]

other gene names :

[RAC1; RAC1; MIG5; Rac-1; TC-25; p21-Rac1; TC25]

uniprot entry name :

RAC1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

211

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF)

app notes :

WB 1:500 - 1:2000. IHC 1:50 - 1:200. IF 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC1 (NP_008839.2). Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products description :

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

ncbi gi num :

9845511

ncbi acc num :

NP_008839.2

ncbi gb acc num :

NM_006908.4

uniprot acc num :

P63000

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Rac Pathway (1270321); Adaptive Immune System Pathway (1269171); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806)

ncbi summary :

uniprot summary :

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, Rho. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: extrinsic to plasma membrane; Golgi membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup. Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; thioesterase binding; Rho GDP-dissociation inhibitor binding; protein kinase binding; Rab GTPase binding. Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; metabolic process; cell motility involved in cell locomotion; regulation of cell migration; small GTPase mediated signal transduction; positive regulation of stress fiber formation; cell adhesion; bone resorption; platelet activation; anatomical structure morphogenesis; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; regulation of defense response to virus by virus; negative regulation of interleukin-23 production; T cell costimulation; cerebral cortex radially oriented cell migration; actin cytoskeleton organization and biogenesis; axon guidance; cell-matrix adhesion; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; ephrin receptor signaling pathway; response to wounding; inflammatory response; regulation of hydrogen peroxide metabolic process; lamellipodium biogenesis; intercellular junction assembly and maintenance; embryonic olfactory bulb interneuron precursor migration; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; G-protein coupled receptor protein signaling pathway; cell proliferation; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage