STK4 Polyclonal Antibody | MyBioSource MBS9127929 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

STK4 Polyclonal Antibody

catalog :

MBS9127929

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using STK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

image 2 :

Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9127929

products type :

Antibody

products full name :

STK4 Polyclonal Antibody

products short name :

[STK4]

other names :

[serine/threonine-protein kinase 4; Serine/threonine-protein kinase 4; serine/threonine-protein kinase 4; serine/threonine kinase 4; Mammalian STE20-like protein kinase 1; MST-1]

products gene name :

[STK4]

other gene names :

[STK4; STK4; KRS2; MST1; YSK3; TIIAC; KRS2; MST1; MST-1; MST1/N; MST1/C]

uniprot entry name :

STK4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

462

purity :

Affinity Purification

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

WB 1:500 - 1:2000. IHC 1:20 - 1:200. IF 1:20 - 1:100. IP 1:20 - 1:50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunoprecipitation (IP)

image4 description :

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody. Western blot was performed from the immunoprecipitate using STK4 antibody at a dilition of 1:1000.

other info1 :

Immunogen: Recombinant Protein

other info2 :

Immunogen: Recombinant protein of human STK4

products description :

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

ncbi gi num :

5454096

ncbi acc num :

NP_006273.1

ncbi gb acc num :

NM_006282.2

uniprot acc num :

Q13043

ncbi mol weight :

56kDa

ncbi pathways :

FoxO Signaling Pathway (921162); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531); Pathways In Cancer (83105); Ras Signaling Pathway (868085); Signal Transduction Pathway (1269379); Signaling By Hippo Pathway (1269616)

ncbi summary :

uniprot summary :

MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage. Protein type: Autophagy; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; MST subfamily. Chromosomal Location of Human Ortholog: 20q11.2-q13.2. Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol. Molecular Function: protein dimerization activity; identical protein binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation. Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage