catalog number :
MBS912792
products type :
ELISA Kit
products full name :
Human Endosialin, CD248 ELISA Kit
products short name :
CD248 molecule, endosialin
products name syn :
Human Endosialin (CD248) ELISA kit; CD164L1; MGC119478; MGC119479; TEM1; 2610111G01Rik; CD164 sialomucin-like 1; CD248 antigen; endosialin; endosialin; tumor endothelial marker 1; CD248 molecule; endosialin
other names :
endosialin; Endosialin; endosialin; 2610111G01Rik; CD164 sialomucin-like 1; CD248 antigen, endosialin; tumor endothelial marker 1; CD248 molecule, endosialin; Tumor endothelial marker 1; CD_antigen: CD248
products gene name :
CD248
other gene names :
CD248; CD248; TEM1; CD164L1; CD164L1; TEM1
uniprot entry name :
CD248_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CD248. No significant cross-reactivity or interference between human CD248 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.31 2 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human CD248 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD248 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD248 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD248 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD248 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_065137.1
ncbi gb acc num :
NM_020404.2
ncbi mol weight :
80,859 Da
uniprot summary :
CD248: May play a role in tumor angiogenesis. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: proteinaceous extracellular matrix; cytoplasm; integral to membrane. Molecular Function: extracellular matrix binding; calcium ion binding; carbohydrate binding. Biological Process: cell migration; positive regulation of cell proliferation; anatomical structure regression; lymph node development
size4 :
10x96-Strip-Wells