HSD3B2 Polyclonal Antibody | MyBioSource MBS9127343 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HSD3B2 Polyclonal Antibody

catalog :

MBS9127343

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using HSD3B2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunofluorescence analysis of A-549 cells using HSD3B2 antibody (MBS9127343)

product information

catalog number :

MBS9127343

products type :

Antibody

products full name :

HSD3B2 Polyclonal Antibody

products short name :

[HSD3B2]

products name syn :

[HSDS3B2 ; HSD3B; HSDB; SDR11E2; 3 beta-hydroxysteroide/Delta 5?isomerase type 2]

other names :

[3 beta-hydroxysteroid dehydrogenase/Delta 5-- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-- 4-isomerase type 2; 3-beta-HSD II; 3 beta-HSD type II; progesterone reductase; delta 5-delta 4-isomerase type II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; short chain dehydrogenase/reductase family 11E, member 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-- 4-isomerase type II; 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal typeIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase]

products gene name :

[HSD3B2]

other gene names :

[HSD3B2; HSD3B2; HSDB; HSD3B; SDR11E2; HSDB3B; 3-beta-HSD II]

uniprot entry name :

3BHS2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

372

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze/ thaw cycles.

tested application :

Western Blot (WB),Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IF 1:10 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence(IF)

other info1 :

Immunogen: Recombinant fusion protein containing a sequene corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1). Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Calculated MW: 24 kDa/42kDa. Observed MW: 47 kDa

products categories :

Polyclonal

products description :

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.

ncbi gi num :

112770

ncbi acc num :

P26439.2

uniprot acc num :

P26439

ncbi pathways :

Androgen Biosynthesis Pathway (106154); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (413383); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); Corticotropin-releasing Hormone Pathway (920957); Glucocorticoid Mineralcorticoid Metabolism Pathway (198902); Glucocorticoid Biosynthesis Pathway (106152); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150)

ncbi summary :

uniprot summary :

HSD3B2: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified. Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.145; Endoplasmic reticulum; Isomerase; Membrane protein, integral; Oxidoreductase; EC 5.3.3.1; Mitochondrial; Lipid Metabolism - C21-steroid hormone. Chromosomal Location of Human Ortholog: 1p13.1. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; mitochondrial membrane; mitochondrial inner membrane; integral to membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane. Molecular Function: steroid delta-isomerase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity. Biological Process: steroid metabolic process; mineralocorticoid biosynthetic process; androgen biosynthetic process; glucocorticoid biosynthetic process; steroid biosynthetic process. Disease: 3-beta-hydroxysteroid Dehydrogenase, Type Ii, Deficiency Of

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage