product summary
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company name :
MyBioSource
product type :
antibody
product name :
UBE3C Polyclonal Antibody
catalog :
MBS9127186
quantity :
0.05 mL
price :
200 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS9127186 image 1
Western blot analysis of extracts of various cell lines, using UBE3C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
image 2 :
MyBioSource MBS9127186 image 2
Immunohistochemistry of paraffin-embedded human breast cancer using UBE3C Antibody at dilution of 1:100 (40x lens).
image 3 :
MyBioSource MBS9127186 image 3
Immunofluorescence analysis of U2OS cells using UBE3C antibody. Blue: DAPI for nuclear staining.
product information
catalog number :
MBS9127186
products type :
Antibody
products full name :
UBE3C Polyclonal Antibody
products short name :
[UBE3C]
products name syn :
[HECTH2]
other names :
[Ubiquitin-protein ligase E3C; Ubiquitin-protein ligase E3C; ubiquitin-protein ligase E3C; ubiquitin-protein isopeptide ligase (E3); ubiquitin protein ligase E3C; HectH2]
products gene name :
[UBE3C]
other gene names :
[UBE3C; UBE3C; HECTH2; KIAA0010; KIAA10]
uniprot entry name :
UBE3C_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human
sequence length :
1083
purity :
Affinity Purification
form :
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
storage stability :
Store at -20°C. Avoid freeze / thaw cycles.
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF 1:10-1:100
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info2 :
Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 1-270 of human UBE3C (NP_055486.2). Calculated MW: 45 kDa/74 kDa/123 kDa. Observed MW: 124 kDa
products categories :
Polyclonal
ncbi gi num :
67462009
ncbi acc num :
Q15386.3
uniprot acc num :
Q15386
ncbi pathways :
Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)
uniprot summary :
UBE3C: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either Lys-29 - or Lys-48 -linked polyubiquitin chains. Has preference for Lys-48 linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 7q36.3. Cellular Component: proteasome complex; cytoplasm; intracellular; nucleus. Molecular Function: protein binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
275
size3 :
0.2 mL
price3 :
430
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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