TREX1 Polyclonal Antibody | MyBioSource MBS9127114 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TREX1 Polyclonal Antibody

catalog :

MBS9127114

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using TREX1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

image 2 :

Immunofluorescence analysis of A549 cells using TREX1 antibody. Blue: DAPI for nuclear staining.

image 3 :

Immunofluorescence analysis of HeLa cells using TREX1 antibody.

product information

catalog number :

MBS9127114

products type :

Antibody

products full name :

TREX1 Polyclonal Antibody

products short name :

[TREX1]

products name syn :

[CRV; AGS1; DRN3; HERNS]

other names :

[Three-prime repair exonuclease 1; Three-prime repair exonuclease 1; three-prime repair exonuclease 1; DNase III; deoxyribonuclease III; 3' repair exonuclease 1; 3'-5' exonuclease TREX1; three prime repair exonuclease 1; 3'-5' exonuclease TREX1; DNase III]

products gene name :

[TREX1]

other gene names :

[TREX1; TREX1; CRV; AGS1; DRN3; HERNS]

uniprot entry name :

TREX1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

369

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20°C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IF: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TREX1(NP_057465.1). Calculated MW: 32kDa/33kDa/38kDa . Observed MW: 37 kDa

products categories :

Polyclonal

products description :

This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants.

ncbi gi num :

47606216

ncbi acc num :

Q9NSU2.1

uniprot acc num :

Q9NSU2

ncbi mol weight :

369

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); G2/M Checkpoints Pathway (105750); G2/M DNA Damage Checkpoint Pathway (105751); IRF3-mediated Induction Of Type I IFN Pathway (833819); Immune System Pathway (106386)

ncbi summary :

uniprot summary :

TREX1: the major 3 DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported. Protein type: Cell cycle regulation; DNA repair, damage; Deoxyribonuclease; EC 3.1.11.2; DNA-binding. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: endoplasmic reticulum membrane; nucleolus; nuclear envelope; nucleus; cytosol. Molecular Function: protein homodimerization activity; metal ion binding; double-stranded DNA binding; 3 -5 -exodeoxyribonuclease activity; exodeoxyribonuclease III activity; MutLalpha complex binding; adenyl deoxyribonucleotide binding; 3 -5 exonuclease activity; MutSalpha complex binding; single-stranded DNA binding. Biological Process: regulation of interferon type I production; mismatch repair; positive regulation of interferon type I production; innate immune response; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; DNA metabolic process; DNA recombination. Disease: Chilblain Lupus 1; Vasculopathy, Retinal, With Cerebral Leukodystrophy; Aicardi-goutieres Syndrome 1; Systemic Lupus Erythematosus

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage