ELISA/assay

Rat Growth arrest-specific protein 6, GAS6 ELISA Kit

MBS912701

48-Strip-Wells

510 USD

ELISA Kit

Rat Growth arrest-specific protein 6, GAS6 ELISA Kit

growth arrest-specific 6

Rat Growth arrest-specific protein 6 (GAS6) ELISA kit; AXLLG; AXSF; DKFZp666G247; FLJ34709; AXL receptor tyrosine kinase ligand; AXL stimulatory factor; growth arrest-specific 6

Growth arrest-specific protein 6; Growth arrest-specific protein 6; growth arrest-specific protein 6; GPF; GAS-6; growth-potentiating factor; AXL receptor tyrosine kinase ligand; growth arrest specific 6; AXL receptor tyrosine kinase ligand; Growth-potentiating factor; GPF

GAS6

Gas6; Gas6; GAS-6; GPF

GAS6_RAT

Rat

674

This assay has high sensitivity and excellent specificity for detection of rat GAS6. No significant cross-reactivity or interference between rat GAS6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.94 ng/ml -60 ng/ml. Sensitivity: The minimum detectable dose of rat GAS6 is typically less than 0.23 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAS6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

48427855

Q63772.1

Q63772

74,638 Da

Cell Surface Interactions At The Vascular Wall Pathway (573932); Gamma-carboxylation Of Protein Precursors Pathway (574336); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (574335); Hemostasis Pathway (573899); Metabolism Of Proteins Pathway (574309); PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (574334); Platelet Activation, Signaling And Aggregation Pathway (573909); Platelet Degranulation Pathway (573926); Post-translational Protein Modification Pathway (574333); Removal Of Aminoterminal Propeptides From Gamma-carboxylated Proteins Pathway (574338)

provides protection of neurons against serum deprivation-induced apoptosis [RGD, Feb 2006]

GAS6: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Ligand, receptor tyrosine kinase; Secreted. Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular. Molecular Function: voltage-gated calcium channel activity; growth factor activity; phosphatidylserine binding; caspase inhibitor activity; protein tyrosine kinase activator activity; calcium ion binding; receptor tyrosine kinase binding; receptor agonist activity; molecular adaptor activity; receptor binding. Biological Process: entry of virus into host cell; tissue remodeling; neuron migration; negative regulation of caspase activity; signal transduction; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of natural killer cell differentiation; positive regulation of protein export from nucleus; positive regulation of fibroblast proliferation; viral envelope fusion with host membrane; negative regulation of protein import into nucleus, translocation; tissue regeneration; positive regulation of glomerular filtration; negative regulation of interferon-gamma production; regulation of growth; positive regulation of phagocytosis; negative regulation of interleukin-6 production; protein kinase B signaling cascade; positive regulation of TOR signaling pathway; virion attachment, binding of host cell surface receptor; organ regeneration; negative regulation of transcription factor activity; activation of protein kinase B; negative regulation of tumor necrosis factor production; viral genome replication; cellular response to starvation; positive regulation of peptidyl-serine phosphorylation; phagocytosis; cell-substrate adhesion; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of protein kinase activity; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of transcription, DNA-dependent; positive regulation of cytokine and chemokine mediated signaling pathway; negative regulation of interleukin-1 secretion; apoptotic cell clearance; negative regulation of apoptosis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800