CD47 Polyclonal Antibody | MyBioSource MBS9126914 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD47 Polyclonal Antibody

catalog :

MBS9126914

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of mouse brain, using CD47 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

image 2 :

Western blot analysis of extracts of various cell lines, using CD47 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

image 3 :

Immunofluorescence analysis of Jurkat cells using CD47 antibody (MBS9126914) at dilution of 1:100. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9126914

products type :

Antibody

products full name :

CD47 Polyclonal Antibody

products short name :

[CD47]

products name syn :

[CD47; IAP; MER6; OA3; CD47 molecule]

other names :

[Leukocyte surface antigen CD47; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 molecule; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47]

products gene name :

[CD47]

other gene names :

[CD47; CD47; IAP; OA3; MER6; MER6; IAP]

uniprot entry name :

CD47_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

other info1 :

Species: Human

other info2 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 oI human CD47 (NP_942088.1).

products categories :

Polyclonal

products description :

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.

ncbi gi num :

1171879

ncbi acc num :

Q08722.1

uniprot acc num :

Q08722

ncbi mol weight :

Calculated MW: 31kDa/33kDa/35kDa. Observed MW: 47kDa

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Integrins In Angiogenesis Pathway (137999); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209); Spinal Cord Injury Pathway (739007)

ncbi summary :

uniprot summary :

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 3q13.1-q13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage