TriMethyl-Histone H3-K56 | MyBioSource MBS9126783 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TriMethyl-Histone H3-K56

catalog :

MBS9126783

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K56 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

product information

catalog number :

MBS9126783

products type :

Antibody

products full name :

TriMethyl-Histone H3-K56

products short name :

[H3-K56]

other names :

[histone H3-K56 acetyltransferase, RTT109, partial; Histone H3.1; histone H3.1; histone H3/j; histone 1, H3j; H3 histone family, member J; histone cluster 1, H3j; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l]

products gene name :

[H3K56me3]

other gene names :

[HIST1H3J; HIST1H3A; H3/j; H3FJ; H3FA]

uniprot entry name :

H31_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat, Other (Wide Range)

sequence length :

146

purity :

Affinity purification

storage stability :

Store at -20 Degree C (regular) or -80 Degree C (long term). Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

app notes :

WB 1:500 - 1:2000. IHC 1:50 - 1:200. IF 1:50 - 1:200. IP 1:50 - 1:200. ChIP 1:20 - 1:100. CHIPseq 1:20 - 1:100

image1 heading :

Western Blot (WB)

other info1 :

Species: Human. Route: Synthetic Peptide. Immunogen : A synthetic peptide of human HIST3H3. Gene ID : 8290. Swiss Prot: Q16695. Synonyms: H3t; H3.4; H3/g; H3FT;

other info2 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Methylation

products description :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

ncbi gi num :

743667947

ncbi acc num :

KID94952.1

ncbi mol weight :

16kDa

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Condensation Of Prophase Chromosomes Pathway (1127547)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

uniprot summary :

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; DNA methylation on cytosine

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage