APTX Polyclonal Antibody | MyBioSource MBS9126730 product information

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company name :

MyBioSource

product type :

antibody

product name :

APTX Polyclonal Antibody

catalog :

MBS9126730

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using APTX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunofluorescence analysis of A549 cells using APTX antibody.

image 3 :

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody. Western blot was performed from the immunoprecipitate using APTX antibody at a dilition of 1:500.

product information

catalog number :

MBS9126730

products type :

Antibody

products full name :

APTX Polyclonal Antibody

products short name :

[APTX]

products name syn :

[AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT]

other names :

[Aprataxin; Aprataxin; aprataxin; forkhead-associated domain histidine triad-like protein; aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT]

products gene name :

[APTX]

other gene names :

[APTX; APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; AXA1; FHA-HIT]

uniprot entry name :

APTX_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

356

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

WB, IF, IP

app notes :

WB: 1:500 - 1:2000. IF: 1:50-1:100. IP 1:20-1:50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunoprecipitation (IP)

other info1 :

Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1). Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Calculated MW: 5kDa/13 kDa/19-40 kDa. Observed MW: 40 kDa.

products categories :

Polyclonal

products description :

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

ncbi gi num :

48428038

ncbi acc num :

Q7Z2E3.2

uniprot acc num :

Q7Z2E3

ncbi summary :

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]

uniprot summary :

APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5 -phosphate termini, resulting in the production of 5 -phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5 - monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleolus; EC 3.-.-.-; C2H2-type zinc finger protein; RNA-binding; Phosphatase (non-protein); DNA repair, damage. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus. Molecular Function: protein binding; DNA 5 -adenosine monophosphate hydrolase activity; phosphoglycolate phosphatase activity; polynucleotide 3 -phosphatase activity; metal ion binding; double-stranded DNA binding; double-stranded RNA binding; damaged DNA binding; phosphoprotein binding; protein N-terminus binding; chromatin binding. Biological Process: single strand break repair; response to hydrogen peroxide; dephosphorylation; double-strand break repair; regulation of protein stability; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; DNA ligation. Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage