ELISA/assay

Human Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit

MBS912664

48-Strip-Wells

510 USD

ELISA Kit

Human Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit

phospholipase A2, group V

Human Calcium-dependent phospholipase A2 (PLA2G5) ELISA kit; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2); Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group V

calcium-dependent phospholipase A2; Calcium-dependent phospholipase A2; calcium-dependent phospholipase A2; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase 5; phospholipase A2, group V; Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5

PLA2G5

PLA2G5; PLA2G5; FRFB; GV-PLA2; PLA2-10; hVPLA(2)

PA2G5_HUMAN

Human

138

This assay has high sensitivity and excellent specificity for detection of Human PLA2G5. No significant cross-reactivity or interference between Human PLA2G5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.18 ng/ml-12 ng/ml. Sensitivity: 0.04 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4505853

NP_000920.1

NM_000929.2

P39877

15,674 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811)

This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family. Protein type: Lipid Metabolism - alpha-linolenic acid; Phospholipase; Secreted; Secreted, signal peptide; Lipid Metabolism - linoleic acid; Cell surface; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 1p36-p34. Cellular Component: Golgi apparatus; cell surface; perinuclear region of cytoplasm; plasma membrane; extracellular region. Molecular Function: heparin binding; calcium-dependent phospholipase A2 activity; calcium ion binding. Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; response to cAMP; phospholipid metabolic process; response to cytokine stimulus; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; lipid catabolic process. Disease: Fleck Retina, Familial Benign

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800