Human Serine/arginine-rich splicing factor 1, SFRS1 ELISA Kit | MyBioSource MBS912663 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Serine/arginine-rich splicing factor 1, SFRS1 ELISA Kit

catalog :

MBS912663

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS912663

products type :

ELISA Kit

products full name :

Human Serine/arginine-rich splicing factor 1, SFRS1 ELISA Kit

products short name :

splicing factor, arginine/serine-rich 1

products name syn :

Human Serine/arginine-rich splicing factor 1 (SFRS1) ELISA kit; ASF; MGC5228; SF2; SF2p33; SRp30a; pre-mRNA-splicing factor SF2; P33 subunit; splicing factor 2; splicing factor; arginine/serine-rich 1

other names :

serine/arginine-rich splicing factor 1 isoform 2; Serine/arginine-rich splicing factor 1; serine/arginine-rich splicing factor 1; splicing factor 2; SR splicing factor 1; alternative-splicing factor 1; pre-mRNA-splicing factor SF2, P33 subunit; splicing factor, arginine/serine-rich, 30-KD, A; serine/arginine-rich splicing factor 1; Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit

products gene name :

SFRS1

other gene names :

SRSF1; SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a; ASF; SF2; SF2P33; SFRS1; OK/SW-cl.3; ASF-1

uniprot entry name :

SRSF1_HUMAN

reactivity :

Human

sequence length :

201

specificity :

This assay has high sensitivity and excellent specificity for detection of human SFRS1. No significant cross-reactivity or interference between human SFRS1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

118582269

ncbi acc num :

NP_001071634.1

ncbi gb acc num :

NM_001078166.1

uniprot acc num :

Q07955

ncbi mol weight :

27,745 Da

ncbi pathways :

Cleavage Of Growing Transcript In The Termination Region Pathway (106560); Gene Expression Pathway (105937); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); RNA Polymerase II Transcription Pathway (106557); RNA Polymerase II Transcription Termination Pathway (106559); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Transport Of Mature Transcript To Cytoplasm Pathway (105959)

ncbi summary :

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

uniprot summary :

SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5 and 3 splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5 -splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. Protein type: Spliceosome; RNA-binding; RNA splicing. Chromosomal Location of Human Ortholog: 17q22. Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus. Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding. Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5 -splice site recognition; mRNA splice site selection; mRNA export from nucleus; regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; gene expression; mRNA 3 -end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage