FBP1 Polyclonal Antibody | MyBioSource MBS9126316 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FBP1 Polyclonal Antibody

catalog :

MBS9126316

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using FBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

image 2 :

Immunofluorescence analysis of MCF-7 cells using FBP1 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9126316

products type :

Antibody

products full name :

FBP1 Polyclonal Antibody

products short name :

[FBP1]

products name syn :

[FBP; FBP1; FBPase 1; fructose 1; 6 bisphosphatase 1]

other names :

[Fructose-1,6-bisphosphatase 1; Fructose-1,6-bisphosphatase 1; fructose-1,6-bisphosphatase 1; FBPase 1; liver FBPase; fructose-bisphosphatase 1; growth-inhibiting protein 17; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; fructose-1,6-bisphosphatase 1; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; Liver FBPase]

products gene name :

[FBP1]

other gene names :

[FBP1; FBP1; FBP; FBP; FBPase 1]

uniprot entry name :

F16P1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

338

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular). Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Species: Human. Immunogen: Recombinant Protein. Category: Primary Antibody

other info2 :

Immunogen: Recombinant protein of human FBP1. Calculated Molecular Weight: 37kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.

ncbi gi num :

311033495

ncbi acc num :

P09467.5

uniprot acc num :

P09467

ncbi mol weight :

338

ncbi pathways :

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

ncbi summary :

uniprot summary :

FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children. Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; EC 3.1.3.11; Carbohydrate Metabolism - fructose and mannose; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Chromosomal Location of Human Ortholog: 9q22.3. Cellular Component: cytoplasm; cytosol. Molecular Function: monosaccharide binding; identical protein binding; protein binding; metal ion binding; fructose-bisphosphatase activity; AMP binding. Biological Process: dephosphorylation; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; glucose metabolic process; regulation of gluconeogenesis; pathogenesis; negative regulation of cell growth; negative regulation of glycolysis; fructose 6-phosphate metabolic process; protein homotetramerization; fructose metabolic process; gluconeogenesis. Disease: Fructose-1,6-bisphosphatase Deficiency

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage