ALYREF Polyclonal Antibody | MyBioSource MBS9126309 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ALYREF Polyclonal Antibody

catalog :

MBS9126309

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using ALYREF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

image 2 :

Immunohistochemistry of paraffin-embedded rat lung using ALYREF antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded rat liver using ALYREF antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS9126309

products type :

Antibody

products full name :

ALYREF Polyclonal Antibody

products short name :

[ALYREF]

products name syn :

[ALY; BEF; REF; THOC4; ALY/REF]

other names :

[THO complex subunit 4; THO complex subunit 4; THO complex subunit 4; tho4; THO complex 4; bZIP enhancing factor; ally of AML-1 and LEF-1; bZIP-enhancing factor BEF; transcriptional coactivator Aly/REF; Aly/REF export factor; Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF]

products gene name :

[ALYREF]

other gene names :

[ALYREF; ALYREF; ALY; BEF; REF; THOC4; ALY/REF; ALY; BEF; THOC4; Tho4]

uniprot entry name :

THOC4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

257

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunoprecipitation (IP)

app notes :

WB: 1:500 - 1:1000

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded rat ovary using ALYREF antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded rat brain using ALYREF antibody at dilution of 1:100 (40x lens).

other info1 :

Species: Human. Category: Primary anitbody

other info2 :

Immunogen: A synthetic peptide of human ALYREF. Calculated Molecular Weight: 26kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

ncbi gi num :

48429165

ncbi acc num :

Q86V81.3

uniprot acc num :

Q86V81

ncbi pathways :

Cleavage Of Growing Transcript In The Termination Region Pathway (106560); Exon Junction Complex (EJC) Pathway (470353); Exon Junction Complex (EJC) Pathway (890638); Gene Expression Pathway (105937); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); RNA Polymerase II Transcription Pathway (106557); RNA Polymerase II Transcription Termination Pathway (106559); RNA Transport Pathway (177876)

ncbi summary :

uniprot summary :

THOC4: a chaperone protein that promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains thus promoting transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Nuclear localization, translocates to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Two differentially spliced isoforms have been described. Protein type: RNA-binding; Spliceosome; Chaperone; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; nuclear speck; cytosol; nucleus. Molecular Function: protein binding; nucleotide binding; single-stranded DNA binding. Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; positive regulation of RNA elongation; regulation of DNA recombination; gene expression; intronless viral mRNA export from host nucleus; mRNA 3 -end processing; termination of RNA polymerase II transcription; replication fork processing

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage