catalog number :
MBS912602
products type :
ELISA Kit
products full name :
Rat Glutathione S-transferase P, GSTP1 ELISA Kit
products short name :
glutathione S-transferase pi 1
products name syn :
Rat Glutathione S-transferase P (GSTP1) ELISA kit; DFN7; FAEES3; GST3; GSTP; PI; deafness; X-linked 7; fatty acid ethyl ester synthase III; glutathione transferase; glutathione S-transferase pi 1
other names :
glutathione S-transferase P; Glutathione S-transferase P; glutathione S-transferase P; GST 7-7; chain 7; GST class-pi; glutathione S-transferase pi 2; glutathione S-transferase, pi 2; glutathione-S-transferase, pi 1; Glutathione-S-transferase placental enzyme pi type; Glutathione-S-transferase, placental enzyme pi type; glutathione S-transferase pi 1; Chain 7; GST 7-7; GST class-pi
products gene name :
GSTP1
other gene names :
Gstp1; Gstp1; Gst3; Gstp; GST-P; Gstp2
uniprot entry name :
GSTP1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat GSTP1. No significant cross-reactivity or interference between Rat GSTP1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 62.5 pg/ml-4000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSTP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSTP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036709.1
ncbi gb acc num :
NM_012577.2
ncbi mol weight :
23,439 Da
ncbi pathways :
Biological Oxidations Pathway 574084!!Diurnally Regulated Genes With Circadian Orthologs Pathway 198473!!Drug Metabolism - Cytochrome P450 Pathway 83424!!Drug Metabolism - Cytochrome P450 Pathway 427!!Glutathione Conjugation Pathway 574109!!Glutathione Metabolism Pathway 83365!!Glutathione Metabolism Pathway 343!!Metabolism Pathway 573935!!Metabolism Of Xenobiotics By Cytochrome P450 Pathway 83423!!Metabolism Of Xenobiotics By Cytochrome P450 Pathway 425
ncbi summary :
may play a role in drug and xenobiotic metabolism [RGD, Feb 2006]
uniprot summary :
Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration . Catalytic activity: RX + glutathione = HX + R-S-glutathione.