Human Syntaxin-1A, STX1A ELISA Kit | MyBioSource MBS912583 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Syntaxin-1A, STX1A ELISA Kit

catalog :

MBS912583

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS912583

products type :

ELISA Kit

products full name :

Human Syntaxin-1A, STX1A ELISA Kit

products short name :

syntaxin 1A (brain)

products name syn :

Human Syntaxin-1A (STX1A) ELISA kit; HPC-1; STX1; p35-1; syntaxin 1A; syntaxin 1A (brain)

other names :

syntaxin-1A isoform 2; Syntaxin-1A; syntaxin-1A; neuron-specific antigen HPC-1; syntaxin 1A (brain); Neuron-specific antigen HPC-1

products gene name :

STX1A

other gene names :

STX1A; STX1A; STX1; HPC-1; P35-1; SYN1A; STX1

uniprot entry name :

STX1A_HUMAN

reactivity :

Human

sequence length :

251

specificity :

This assay has high sensitivity and excellent specificity for detection of Human STX1A. No significant cross-reactivity or interference between Human STX1A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Cycle. Protein Biological Process 3: Exocytosis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for STX1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STX1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

259906442

ncbi acc num :

NP_001159375.1

ncbi gb acc num :

NM_001165903.1

uniprot acc num :

Q16623

ncbi mol weight :

33,023 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Botulinum Neurotoxicity Pathway (105734); Diabetes Pathways (105902); Disease Pathway (530764); Dopamine Neurotransmitter Release Cycle Pathway (106523); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174); Glutamate Neurotransmitter Release Cycle Pathway (106522)

ncbi summary :

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

uniprot summary :

STX1A: a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described. Protein type: Vesicle; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: SNARE complex; synaptic vesicle; neuron projection; synaptic vesicle membrane; extracellular region; plasma membrane; integral to membrane; endomembrane system; actomyosin; cytosol; cell junction; secretory granule. Molecular Function: protein binding, bridging; protein domain specific binding; SNAP receptor activity; myosin binding; protein N-terminus binding; calcium-dependent protein binding; ATP-dependent protein binding; SNARE binding; protein binding; chloride channel inhibitor activity; protein heterodimerization activity; myosin head/neck binding; calcium channel inhibitor activity; glycoprotein binding; kinase binding. Biological Process: response to gravity; neurotransmitter secretion; pathogenesis; positive regulation of neurotransmitter secretion; calcium ion-dependent exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic transmission; intracellular protein transport; vesicle docking; cellular protein metabolic process; glutamate secretion; energy reserve metabolic process; regulation of insulin secretion; positive regulation of exocytosis; synaptic vesicle docking during exocytosis; secretion by cell

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage