Human Guanylate cyclase soluble subunit alpha-3, GUCY1A3 ELISA Kit | MyBioSource MBS912571 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Guanylate cyclase soluble subunit alpha-3, GUCY1A3 ELISA Kit

catalog :

MBS912571

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS912571

products type :

ELISA Kit

products full name :

Human Guanylate cyclase soluble subunit alpha-3, GUCY1A3 ELISA Kit

products short name :

guanylate cyclase 1, soluble, alpha 3

products name syn :

Human Guanylate cyclase soluble subunit alpha-3 (GUCY1A3) ELISA kit; GC-SA3; GUC1A3; GUCA3; GUCSA3; GUCY1A1; GC-S-alpha-1; soluble guanylate cyclase large subunit; guanylate cyclase 1; soluble; alpha 3

other names :

guanylate cyclase soluble subunit alpha-3 isoform A; Guanylate cyclase soluble subunit alpha-3; guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; GC-S-alpha-1; soluble guanylate cyclase large subunit; guanylate cyclase 1, soluble, alpha 3; GCS-alpha-1; Soluble guanylate cyclase large subunit

products gene name :

GUCY1A3

other gene names :

GUCY1A3; GUCY1A3; GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A1; GUC1A3; GUCSA3; GUCY1A1; GCS-alpha-3

uniprot entry name :

GCYA3_HUMAN

reactivity :

Human

sequence length :

690

specificity :

This assay has high sensitivity and excellent specificity for detection of Human GUCY1A3. No significant cross-reactivity or interference between Human GUCY1A3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Adenosine synthesis and metabolism. Protein Biological Process 3: cGMP biosynthesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GUCY1A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GUCY1A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUCY1A3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUCY1A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

67763816

ncbi acc num :

NP_000847.2

ncbi gb acc num :

NM_000856.5

uniprot acc num :

Q02108

ncbi mol weight :

77,452 Da

ncbi pathways :

Gap Junction Pathway (83072); Gap Junction Pathway (483); Hemostasis Pathway (106028); Long-term Depression Pathway (83086); Long-term Depression Pathway (497); Myometrial Relaxation And Contraction Pathways (198759); Nitric Oxide Stimulates Guanylate Cyclase Pathway (187200); Platelet Homeostasis Pathway (187198); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307)

ncbi summary :

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

GUCY1A3: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3 ,5 -cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]. Protein type: Nucleotide Metabolism - purine; Lyase; EC 4.6.1.2; Receptor, misc.; Guanylyl cyclase. Chromosomal Location of Human Ortholog: 4q31.1-q31.2. Cellular Component: guanylate cyclase complex, soluble. Molecular Function: guanylate cyclase activity; GTP binding; heme binding; receptor activity. Biological Process: cGMP biosynthetic process; relaxation of vascular smooth muscle; positive regulation of cGMP biosynthetic process; blood circulation; regulation of blood pressure; blood coagulation; response to defense-related host nitric oxide production; nitric oxide mediated signal transduction. Disease: Moyamoya Disease 6 With Achalasia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage