catalog number :
MBS912513
products type :
ELISA Kit
products full name :
Mouse Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE1 ELISA Kit
products short name :
lymphatic vessel endothelial hyaluronan receptor 1
products name syn :
Mouse Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE1) ELISA kit; CRSBP-1; HAR; LYVE-1; XLKD1; cell surface retention sequence binding protein-1; extracellular link domain containing 1; extracellular link domain-containing 1; hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1
other names :
lymphatic vessel endothelial hyaluronic acid receptor 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; extra cellular link domain-containing 1; lymphatic vessel endothelial HA recptor-1; lymphatic vessel endothelial HA receptor-1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; lymphatic vessel endothelial hyaluronan receptor 1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1
products gene name :
LYVE1
other gene names :
Lyve1; Lyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik; Crsbp1; Xlkd1; LYVE-1; CRSBP-1
uniprot entry name :
LYVE1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LYVE1. No significant cross-reactivity or interference between mouse LYVE1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: The minimum detectable dose of mouse LYVE1 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LYVE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LYVE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LYVE1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LYVE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_444477.2
ncbi gb acc num :
NM_053247.4
ncbi mol weight :
34,574 Da
ncbi pathways :
Glycosaminoglycan Metabolism Pathway (639874); Hyaluronan Metabolism Pathway (639875); Hyaluronan Uptake And Degradation Pathway (639877); Metabolism Pathway (639859); Metabolism Of Carbohydrates Pathway (639860)
uniprot summary :
XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral. Cellular Component: membrane; plasma membrane; integral to membrane. Molecular Function: transmembrane receptor activity; hyaluronic acid binding. Biological Process: glycosaminoglycan catabolic process; transport; signal transduction; cell adhesion
size4 :
10x96-Strip-Wells