Human Arginase-1, ARG1 ELISA Kit | MyBioSource MBS912500 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Arginase-1, ARG1 ELISA Kit

catalog :

MBS912500

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS912500

products type :

ELISA Kit

products full name :

Human Arginase-1, ARG1 ELISA Kit

products short name :

arginase, liver

products name syn :

Human Arginase-1 (ARG1) ELISA kit; arginase 1; liver-type arginase; type I arginase; arginase; liver

other names :

arginase-1 isoform 2; Arginase-1; arginase-1; arginase, liver; type I arginase; liver-type arginase; arginase 1; Liver-type arginase; Type I arginase

products gene name :

ARG1

other gene names :

ARG1; ARG1

uniprot entry name :

ARGI1_HUMAN

reactivity :

Human

sequence length :

322

specificity :

This assay has high sensitivity and excellent specificity for detection of human ARG1. No significant cross-reactivity or interference between human ARG1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, ascitic fluid, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

10947139

ncbi acc num :

NP_000036.2

ncbi gb acc num :

NM_000045.3

uniprot acc num :

P05089

ncbi mol weight :

34,735 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); IL4-mediated Signaling Events Pathway (137933); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (413403); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (468321)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus. Molecular Function: manganese ion binding; arginase activity. Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; response to amine stimulus; urea cycle; lung development. Disease: Argininemia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage