ELISA/assay

Hamster adiponectin, C1Q and collagen domain containing, ADIPOQ ELISA Kit

MBS912278

96 Strip Wells

765 USD

ELISA Kit

Hamster adiponectin, C1Q and collagen domain containing, ADIPOQ ELISA Kit

adiponectin, C1Q and collagen domain containing (ADIPOQ)

Hamster adiponectin; C1Q and collagen domain containing (ADIPOQ) ELISA kit; Hamster adiponectin; C1Q and collagen domain containing (ADIPOQ)

adiponectin; Adiponectin; adiponectin; apM-1; adipocyte complement related 30kDa protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte complement related protein of 30 kDa; adipose most abundant gene transcript 1 protein; adipocyte, C1q and collagen domain-containing protein; adiponectin, C1Q and collagen domain containing<; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1

ADIPOQ

ADIPOQ; ADIPOQ; APM1; ACRP30; ACRP30; APM1; ACRP30; apM-1

ADIPO_BOVIN

Hamster

240

This assay has high sensitivity and excellent specificity for detection of Hamster ADIPOQ. No significant cross-reactivity or interference between Hamster ADIPOQ and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.469 ng/ml-30 ng/ml. Sensitivity: 0.117 ng/ml

Protein Biological Process 1: Metabolism

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADIPOQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADIPOQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADIPOQ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADIPOQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

27807433

NP_777167.1

NM_174742.2

Q3Y5Z3

26,133 Da

Adipocytokine Signaling Pathway 84332!!Adipocytokine Signaling Pathway 505!!PPAR Signaling Pathway 84281!!PPAR Signaling Pathway 450!!Type II Diabetes Mellitus Pathway 84333!!Type II Diabetes Mellitus Pathway 506

Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW

96 Strip Wells

765 USD