ELISA/assay

Human Myocilin, MYOC ELISA Kit

MBS912080

48-Strip-Wells

510 USD

ELISA Kit

Human Myocilin, MYOC ELISA Kit

myocilin, trabecular meshwork inducible glucocorticoid response

Human Myocilin (MYOC) ELISA kit; GLC1A; GPOA; JOAG; JOAG1; TIGR; myocilin; mutated trabecular meshwork-induced glucocorticoid response protein; myocilin; myocilin; trabecular meshwork inducible glucocorticoid response

myocilin; Myocilin; myocilin; myocilin 55 kDa subunit; mutated trabecular meshwork-induced glucocorticoid response protein; myocilin, trabecular meshwork inducible glucocorticoid response; Myocilin 55 kDa subunit; Trabecular meshwork-induced glucocorticoid response proteinCleaved into the following 2 chains:Myocilin, N-terminal fragmentAlternative name(s):Myocilin 20 kDa N-terminal fragment

MYOC

MYOC; MYOC; GPOA; JOAG; TIGR; GLC1A; JOAG1; myocilin; GLC1A; TIGR

MYOC_HUMAN

Human

504

This assay has high sensitivity and excellent specificity for detection of human MYOC. No significant cross-reactivity or interference between human MYOC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 U/ml -10 U/ml. Sensitivity: The minimum detectable dose of human MYOC is typically less than 0.039 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYOC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYOC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYOC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYOC bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557779

NP_000252.1

NM_000261.1

Q99972

56,972 Da

MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq, Jul 2008]

MYOC: May participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma type 1A (GLC1A). Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Defects in MYOC are a cause of primary congenital glaucoma type 3A (GLC3A). An autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early choldhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. MYOC variations may contribute to GLC3A via digenic inheritance with CYP1B1 and/or another locus associated with the disease. Protein type: Secreted; Endoplasmic reticulum; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23-q24. Cellular Component: Golgi apparatus; extracellular matrix; extracellular space; proteinaceous extracellular matrix; mitochondrial outer membrane; rough endoplasmic reticulum; endoplasmic reticulum; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; mitochondrial intermembrane space; cytoplasmic vesicle; cilium. Molecular Function: protein binding; frizzled binding; fibronectin binding; myosin light chain binding; receptor tyrosine kinase binding. Biological Process: clustering of voltage-gated sodium channels; negative regulation of stress fiber formation; negative regulation of cell-matrix adhesion; skeletal muscle hypertrophy; myelination in the peripheral nervous system; positive regulation of phosphoinositide 3-kinase cascade; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of mitochondrial depolarization; positive regulation of focal adhesion formation; negative regulation of Rho protein signal transduction; regulation of MAPKKK cascade; positive regulation of stress fiber formation; neurite development; positive regulation of cell migration. Disease: Glaucoma 1, Open Angle, A

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800