ELISA/assay

Human Serpin B5, SERPINB5 ELISA Kit

MBS911955

48-Strip-Wells

510 USD

ELISA Kit

Human Serpin B5, SERPINB5 ELISA Kit

serpin peptidase inhibitor, clade B (ovalbumin) , member 5

Human Serpin B5 (SERPINB5) ELISA kit; PI5; maspin; protease inhibitor 5 (maspin) ; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5; serpin peptidase inhibitor; clade B (ovalbumin); member 5

serpin B5; Serpin B5; serpin B5; PI-5; peptidase inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; Maspin; Peptidase inhibitor 5; PI-5

SERPINB5

SERPINB5; SERPINB5; PI5; maspin; PI5; PI-5

SPB5_HUMAN

Human

375

This assay has high sensitivity and excellent specificity for detection of human SERPINB5. No significant cross-reactivity or interference between human SERPINB5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SERPINB5 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

167860126

NP_002630.2

NM_002639.4

P36952

42,100 Da

ATF-2 Transcription Factor Network Pathway (138006); Direct P53 Effectors Pathway (137939); P53 Signaling Pathway (83055); P53 Signaling Pathway (465)

maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 18q21.33. Cellular Component: extracellular space; cytoplasm. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding. Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800