ELISA/assay

Mouse Chitinase-3-like protein 1, CHI3L1 ELISA Kit

MBS911948

48-Strip-Wells

510 USD

ELISA Kit

Mouse Chitinase-3-like protein 1, CHI3L1 ELISA Kit

chitinase 3-like 1 (cartilage glycoprotein-39)

Mouse Chitinase-3-like protein 1 (CHI3L1) ELISA kit; ASRT7; DKFZp686N19119; FLJ38139; GP39; HC-gp39; HCGP-3P; YKL40; YYL-40; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39)

chitinase-3-like protein 1; Chitinase-3-like protein 1; chitinase-3-like protein 1; GP-39; CGP-39; chitinase 3-like 1; cartilage glycoprotein 39; breast regression protein 39; chitinase-like 1; BRP39 protein; Breast regression protein 39; Cartilage glycoprotein 39; CGP-39; GP-39

CHI3L1

Chil1; Chi3l1; Gp39; Brp39; Chi3l1; AW208766; Brp39; Chil1; CGP-39; GP-39

CH3L1_MOUSE

Mouse

389

This assay has high sensitivity and excellent specificity for detection of Mouse CHI3L1. No significant cross-reactivity or interference between Mouse CHI3L1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.47 ng/ml-30 ng/ml. Sensitivity: 0.11 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHI3L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHI3L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHI3L1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

224809461

NP_031721.2

NM_007695.3

Q61362

43,893 Da

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family. Protein type: Secreted, signal peptide; Cell adhesion; Secreted. Cellular Component: extracellular space; endoplasmic reticulum; cytoplasm; extracellular region. Molecular Function: chitin binding; chitinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds. Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; apoptosis; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800