catalog number :
MBS911919
products type :
ELISA Kit
products full name :
Pig Granulocyte-macrophage colony-stimulating factor, CSF2 ELISA Kit
products short name :
colony stimulating factor 2 (granulocyte-macrophage)
products name syn :
Pig Granulocyte-macrophage colony-stimulating factor (CSF2) ELISA kit; GMCSF; MGC131935; MGC138897; colony stimulating factor 2; granulocyte-macrophage colony stimulating factor; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage)
other names :
granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; Colony-stimulating factor; CSF
products gene name :
CSF2
other gene names :
CSF2; CSF2; GM-CSF; GM-CSF; CSF
uniprot entry name :
CSF2_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig CSF2. No significant cross-reactivity or interference between Pig CSF2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_999283.1
ncbi gb acc num :
NM_214118.2
ncbi mol weight :
16,254 Da
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493); HTLV-I Infection Pathway (373897); HTLV-I Infection Pathway (373889); Hematopoietic Cell Lineage Pathway (84490); Hematopoietic Cell Lineage Pathway (489)
uniprot summary :
Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes
size4 :
10x96-Strip-Wells