product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Gamma-glutamyl hydrolase, GGH ELISA Kit
catalog :
MBS911794
quantity :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS911794
products type :
ELISA Kit
products full name :
Human Gamma-glutamyl hydrolase, GGH ELISA Kit
products short name :
gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
products name syn :
Human Gamma-glutamyl hydrolase (GGH) ELISA kit; GH; conjugase; folylpolygammaglutamyl hydrolase; gamma-glutamyl hydrolase; gamma-glutamyl hydrolase (conjugase; folylpolygammaglutamyl hydrolase)
other names :
gamma-glutamyl hydrolase; Gamma-glutamyl hydrolase; gamma-glutamyl hydrolase; gamma-Glu-X carboxypeptidase; gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); Conjugase; GH; Gamma-Glu-X carboxypeptidase
products gene name :
GGH
other gene names :
GGH; GGH; GH
uniprot entry name :
GGH_HUMAN
reactivity :
Human
sequence length :
318
specificity :
This assay has high sensitivity and excellent specificity for detection of human GGH. No significant cross-reactivity or interference between human GGH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human gamma-glutamyl hydrolase (GGH) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 0.45 ng/ml -30 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of human GGH is typically less than 0.11 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GGH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GGH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GGH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GGH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
4503987
ncbi acc num :
NP_003869.1
ncbi gb acc num :
NM_003878.2
uniprot acc num :
Q92820
ncbi mol weight :
35,964 Da
ncbi pathways :
Fluoropyrimidine Activity Pathway 198778!!Folate Biosynthesis Pathway 83018!!Folate Biosynthesis Pathway 404!!Glutamate Removal From Folates Pathway 142235!!Glutathione-mediated Detoxification Pathway 142435
ncbi summary :
This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Catalytic activity: Hydrolysis of a gamma-glutamyl bond.
size :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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