Mouse Protein argonaute-2, EIF2C2 ELISA Kit | MyBioSource MBS911752 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Protein argonaute-2, EIF2C2 ELISA Kit

catalog :

MBS911752

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911752

products type :

ELISA Kit

products full name :

Mouse Protein argonaute-2, EIF2C2 ELISA Kit

products short name :

eukaryotic translation initiation factor 2C, 2

products name syn :

Mouse Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; eukaryotic translation initiation factor 2C; 2

other names :

protein argonaute-2; Protein argonaute-2; protein argonaute-2; mAgo2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; argonaute RISC catalytic component 2; Piwi/Argonaute family protein meIF2C2; eukaryotic translation initiation factor 2C, 2; argonaute RISC catalytic subunit 2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; Piwi/argonaute family protein meIF2C2; Protein slicer

products gene name :

EIF2C2

other gene names :

Ago2; Ago2; Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493; Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2

uniprot entry name :

AGO2_MOUSE

reactivity :

Mouse

sequence length :

860

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse EIF2C2. No significant cross-reactivity or interference between mouse EIF2C2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse EIF2C2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF2C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF2C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF2C2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF2C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

219842353

ncbi acc num :

NP_694818.3

ncbi gb acc num :

NP_694818.3

uniprot acc num :

Q8CJG0

ncbi mol weight :

97,304 Da

ncbi pathways :

Gene Expression Pathway (574654); MicroRNA (miRNA) Biogenesis Pathway (574719); Post-transcriptional Silencing By Small RNAs Pathway (574721); Regulatory RNA Pathways (574718); Small Interfering RNA (siRNA) Biogenesis Pathway (574720)

uniprot summary :

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi). Protein type: RNA processing; RNA-binding; EC 3.1.26.n2. Cellular Component: polysome; mRNA cap complex; membrane; cytoplasm; dendrite; nucleus; cytosol; ribonucleoprotein complex. Molecular Function: mRNA binding; endoribonuclease activity; protein binding; siRNA binding; nuclease activity; miRNA binding; nucleic acid binding; hydrolase activity; RNA binding; endonuclease activity; metal ion binding; RNA 7-methylguanosine cap binding. Biological Process: regulation of translation; negative regulation of translational initiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pre-microRNA processing; multicellular organismal development; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; RNA-mediated gene silencing; cell differentiation; post-embryonic development; mRNA cleavage

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage