Human DNA-binding protein inhibitor ID-1, ID1 ELISA Kit | MyBioSource MBS911748 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DNA-binding protein inhibitor ID-1, ID1 ELISA Kit

catalog :

MBS911748

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911748

products type :

ELISA Kit

products full name :

Human DNA-binding protein inhibitor ID-1, ID1 ELISA Kit

products short name :

inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

products name syn :

Human DNA-binding protein inhibitor ID-1 (ID1) ELISA kit; ID; bHLHb24; DNA-binding protein inhibitor ID-1; dJ857M17.1.2 (inhibitor of DNA binding 1; dominant negative helix-loop-helix protein) ; inhibitor of DNA binding 1; inhibitor of differentiation 1; inhibitor of DNA binding 1; dominant negative helix-loop-helix protein

other names :

DNA-binding protein inhibitor ID-1 isoform a; DNA-binding protein inhibitor ID-1; DNA-binding protein inhibitor ID-1; inhibitor of differentiation 1; class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; Inhibitor of differentiation 1

products gene name :

ID1

other gene names :

ID1; ID1; ID; bHLHb24; BHLHB24; ID; bHLHb24

uniprot entry name :

ID1_HUMAN

reactivity :

Human

sequence length :

155

specificity :

This assay has high sensitivity and excellent specificity for detection of human ID1. No significant cross-reactivity or interference between human ID1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ID1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31317299

ncbi acc num :

NP_002156.2

ncbi gb acc num :

NM_002165.3

uniprot acc num :

P41134

ncbi mol weight :

16,133 Da

ncbi pathways :

ALK1 Signaling Events Pathway (137968); IL-3 Signaling Pathway (198881); Id Signaling Pathway (198871); Notch-mediated HES/HEY Network Pathway (169347); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

ncbi summary :

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 20q11. Cellular Component: nucleoplasm; Golgi apparatus; nucleus. Molecular Function: protein dimerization activity; protein binding; transcription factor activity. Biological Process: circadian rhythm; transcription, DNA-dependent; collagen metabolic process; protein destabilization; blood vessel endothelial cell migration; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; response to antibiotic; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage