Human Coagulation factor XIII B chain, F13B ELISA Kit | MyBioSource MBS911692 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Coagulation factor XIII B chain, F13B ELISA Kit

catalog :

MBS911692

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911692

products type :

ELISA Kit

products full name :

Human Coagulation factor XIII B chain, F13B ELISA Kit

products short name :

coagulation factor XIII, B polypeptide

products name syn :

Human Coagulation factor XIII B chain (F13B) ELISA kit; FXIIIB; TGase; coagulation factor XIII B subunit; coagulation factor XIII; B polypeptide

other names :

coagulation factor XIII B chain; Coagulation factor XIII B chain; coagulation factor XIII B chain; TGase; transglutaminase B chain; fibrin-stabilizing factor B subunit; protein-glutamine gamma-glutamyltransferase B chain; coagulation factor XIII, B polypeptide; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

products gene name :

F13B

other gene names :

F13B; F13B; FXIIIB

uniprot entry name :

F13B_HUMAN

reactivity :

Human

sequence length :

661

specificity :

This assay has high sensitivity and excellent specificity for detection of Human F13B. No significant cross-reactivity or interference between Human F13B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F13B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F13B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

110611237

ncbi acc num :

NP_001985.2

ncbi gb acc num :

NM_001994.2

uniprot acc num :

P05160

ncbi mol weight :

75,511 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Steroid Biosynthesis Pathway (198866)

ncbi summary :

This gene encodes coagulation factor XIII B subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

uniprot summary :

F13B: The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Defects in F13B are the cause of factor XIII subunit B deficiency (FA13BD). FA13BD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q31-q32.1. Cellular Component: extracellular region. Biological Process: blood coagulation. Disease: Factor Xiii, B Subunit, Deficiency Of

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage